The Tfact p53 (Phospho-Ser20) TFact™ DNA-Binding ELISA components necessary for detection and qualitative analysis of p53 (Phospho-Ser20) active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ p53 (Phospho-Ser20) DNA-Binding ELISA detects active p53 (Phospho-Ser20) in HepG2 and MCF7 Nuclear Extract. The HepG2 and MCF7 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.