The Tfact p53 (Phospho-Ser376) TFact™ DNA-Binding ELISA components necessary for detection and qualitative analysis of p53 (Phospho-Ser376) active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ p53 (Phospho-Ser376) DNA-Binding ELISA detects active p53 (Phospho-Ser376) in Hela, MCF7 and HepG2 Nuclear Extract. The Hela, MCF7 and HepG2 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.