The RARα (Phospho-Ser77) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect RARα (Phospho-Ser77) protein expression profile in cells. The kit can be used for measuring the relative amounts of RARα (Phospho-Ser77) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on RARα (Phospho-Ser77).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2032
Format
Two 96-Well Plates
Detection Target
RARα (Phospho-Ser77)
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
P10276
NCBI Gene ID
5914
Target Synonyms
RARA; NR1B1; Retinoic acid receptor alpha; RAR-alpha; Nuclear receptor subfamily 1 group B member 1
Western blot analysis of lysates from HeLa cells, using Retinoic Acid Receptor alpha Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30' and Jurkat cells treated with insulin 0.01U/ml 15', using Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody. The lane on the right is blocked with the phospho peptide.