The Tfact p53 TFact™ DNA-Binding ELISA contains components necessary for detection and qualitative analysis of p53 active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
The TFact™ p53 DNA-Binding ELISA detects active p53 in MCF7 and HepG2 Nuclear Extract. The MCF7 and HepG2 cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract.
