IRS-1 (Phospho-Ser639) Cell-Based ELISA Kit (CBP2082) | Assay Biotech Inc.

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IRS-1 (Phospho-Ser639) Colorimetric Cell-Based ELISA Kit

IRS-1 (Phospho-Ser639) Colorimetric Cell-Based ELISA Kit

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: CBP2082

Reactivity:Human,Mouse,Rat

Assay Type:Cell-Based ELISA

Format:Two 96-Well Plates

Range:At least 5000 cells/well

Pairing Option:Non-Phospho Antibody

Size:

Price/Quantity $500

Quantity:

Datasheet

SDS

Crystal Violet Normalization

Customizable Format

Buy One Get One

IRS-1 (Phospho-Ser639) Colorimetric Cell-Based ELISA Kit

An illustrated breakdown of the Colorimetric Cell-Based ELISA assay principles.

Delivery

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Lead Time

Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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Telephone

408-747-0185

Fax

408-747-0145

Email

order@assaybiotech.com

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Product Details

Product Name

IRS-1 (Phospho-Ser639) Colorimetric Cell-Based ELISA Kit

Description

The IRS-1 (Phospho-Ser639) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect IRS-1 (Phospho-Ser639) protein expression profile in cells. The kit can be used for measuring the relative amounts of IRS-1 (Phospho-Ser639) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on IRS-1 (Phospho-Ser639). This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.

Catalog No

CBP2082

Format

Two 96-Well Plates

Detection Target

IRS-1 (Phospho-Ser639)

Reactivity

Human, Mouse, Rat

Detection Method

Colorimetric 450 nm

Assay Type

Cell-Based ELISA

SwissProt ID

P35568

NCBI Gene ID

3667

Target Synonyms

IRS1; Insulin receptor substrate 1; IRS-1

Storage and Stability Guarantee

4°C/6 Months

Sample Type

Researchers plate their cell line of choice

Range

At least 5000 cells/well

Time

4.5 hours

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Primary Antibodies

Unmodified/Total Antibodies

Phospho Specific Antibodies

Cleaved Specific Antibodies

Methyl Specific Antibodies

Acetyl Specific Antibodies

Nano Antibodies

COVID Antibodies

Monoclonal Antibodies

ELISA Kits

Colorimetric Cell-Based Phospho

Colorimetric Non-Phospho Cell-Based ELISA

Fluorometric Phospho Cell-Based ELISA

Colorimetric Phospho Sandwich ELISA

Chemiluminescent Sandwich ELISA

Colorimetric Non-Phospho Transcription Factor ELISA

Colorimetric Phospho Transcription Factor ELISA

Colorimetric Cell-Based Methyl

Colorimetric Cell-Based Cleaved

Colorimetric Cell-Based Acetyl

Colorimetric Sandwich ELISA

Research Areas

Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis