This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]
Catalog No
A8220
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
IRS1; Insulin receptor substrate 1; IRS-1
Molecular Weight (Da)
131591
Gene Name
IRS1
Protein Name
Insulin receptor substrate 1
Human Gene ID
3667
Human Swiss Prot No.
P35568
Immunogen
The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Tyr896. AA range:862-911
Specificity
Phospho-IRS-1 (Y896) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Y896.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using IRS-1 (Phospho-Tyr896) Antibody. The lane on the right is blocked with the phospho peptide.
