The Phospho-IRS-1(Ser312) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-IRS-1(Ser312) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-IRS-1(Ser312) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-IRS-1(Ser312).