The Akt (Phospho-Thr308) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Akt (Phospho-Thr308) protein expression profile in cells. The kit can be used for measuring the relative amounts of Akt (Phospho-Thr308) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Akt (Phospho-Thr308).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2048
Format
Two 96-Well Plates
Detection Target
Akt (Phospho-Thr308)
Reactivity
Human, Mouse, Rat, Monkey
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
P31749/P31751/Q9Y243
NCBI Gene ID
207/208/10000
Target Synonyms
AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B
Western blot analysis of lysates from Jurkat cells, treated with H2O2, using Akt Antibody. The lane on the right is blocked with the synthesized peptide.
