The Akt (Phospho-Ser473) Phospho Sandwich ELISA Kit allows for qualitative detection of Akt (Phospho-Ser473) in Cell Culture lysate samples in the range of >16 μg/mL Total Protein
HeLa cells were grown to 90% confluency and were stimulated with EGF 200 ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of Akt P-Ser473 versus untreated HeLa lysates.