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Akt Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: B8075
Reactivity:Human,Mouse,Rat,Chicken
Applications:IF,WB,IHC,ELISA
Modification:Unmodified/Total
Source:Polyclonal,Rabbit,IgG
Storage and Stability:Store at -20°C/1 year

$205
Product Details
Product Name
Akt Antibody
Description
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Catalog No
B8075
Reactivity
Human, Mouse, Rat, Chicken
Source
Polyclonal, Rabbit,IgG
Modification
Unmodified/Total
Applications
IF, WB, IHC, ELISA
Dilution
IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B
Molecular Weight (Da or kDa)
Gene Name
AKT1/AKT2/AKT3
Protein Name
RAC-alpha serine/threonine-protein kinase/RAC-beta serine/threonine-protein kinase/RAC-gamma serine/threonine-protein kinase
Human Gene ID
207/208/10000
Human Swiss Prot No.
P31749 / P31751 / Q9Y243
Immunogen
The antiserum was produced against synthesized peptide derived from human AKT1/2/3. AA range:281-330
Specificity
Akt Polyclonal Antibody detects endogenous levels of Akt protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (70)