Akt Antibody

Western blot analysis of lysates from HeLa cells, using Akt Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Akt Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of KB A549 using Akt Polyclonal Antibody diluted at 1:2000

Western Blot analysis of HeLa cells using Akt Polyclonal Antibody diluted at 1:2000

The picture was kindly provided by our customer

Western Blot analysis of various cells using Akt Polyclonal Antibody diluted at 1:2000

Western Blot analysis of 3T3 cells treated with PDGF using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)

Western blot analysis of lysates from 1) 3T3, 2) HepG2,3) Hela,4) A549 cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:SA0439)was diluted at 1:10000, 37° 1hour. （Red） GAPDH Monoclonal Antibody(2B8) (cat:N1293) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:SA0436)was diluted at 1:10000, 37° 1hour.

Western blot analysis of lysates from A431 and Hela cells treated with UV or insulin 0.01U/ml, using AKT Antibody. Primary Antibody was diluted at 1:1000 4° over night,secondary antibody(ABT cat:SA0439)was diluted at 1:10000, 37° 1hour.

Wang, Bin, et al. "Loss of Tctn3 causes neuronal apoptosis and neural tube defects in mice." Cell death & disease 9.5 (2018): 520.