coactivator associated arginine methyltransferase 1(CARM1) Homo sapiens. This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9. [provided by RefSeq, Aug 2013],
Catalog No
N0534
Reactivity
Human,Rat,Monkey
Applications
WB,IHC-p,IF/ICC,FCM,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Immunofluorescence analysis of Hela cells using PRMT4 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) with DAB staining using PRMT4 Monoclonal Antibody.
Western Blot analysis using PRMT4 Monoclonal Antibody against MCF-7 (1). HeLa (2). NIH/3T3 (3). HL-60 (4). LNcap (5). Jurkat (6). PC-3 (7). Cos7 (8). and PC-12 (9) cell lysate.
