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> Products > Primary Antibodies > GAPDH Monoclonal Antibody(2B8)

GAPDH Monoclonal Antibody(2B8)

Overview Description
Datasheet SDS
   Signaling Intermediates   
Catalog No : N1293
  • Reactivity
    Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Sheep,Insect,Yeast
  • Applications
    WB,IF/ICC,IHC-p
  • Modification
    Unmodfied/ Total
  • Source
    Monoclonal Mouse
  • Storage and Stability
    -20°C/1 year
  • Size
    100 ul
  • Price/Quantity
    $200
  • Quantity
Add to Cart
Details
  • Product Name
    GAPDH Monoclonal Antibody(2B8)
  • Description
    glyceraldehyde-3-phosphate dehydrogenase(GAPDH)Homo sapiens. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri
  • Catalog No
    N1293
  • Reactivity
    Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Sheep,Insect,Yeast
  • Applications
    WB,IF/ICC,IHC-p
  • Modification
    Unmodfied/ Total
  • Source
    Monoclonal Mouse
  • Dilution
    WB: 1:5000-20000 IHC: 1:200-300 IF 1:200
  • Purification
    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
  • Concentration
  • Storage and Stability
    -20°C/1 year
  • Other Name
    GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
  • Molecular Weight (Da)
  • Gene Name
    GAPDH
  • Protein Name
    Glyceraldehyde-3-phosphate dehydrogenase
  • Human Gene ID
    2597
  • Human Swiss Prot No.
    P04406
  • Immunogen
    Synthetic Peptide of GAPDH
  • Specificity
    The antibody detects endogenous GAPDH protein.
  • Formulation
    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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Application Data
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Application Data
The picture was kindly provided by our customer
Application Data
The picture was kindly provided by our customer
Application Data
Western blot analysis of Hela (1). Rat brain (2). Rabbit Muscle (3). Sheep Muscle (4). and Mouse brain (5). diluted at 1:10000.
Application Data
Immunofluorescence analysis of Mouse-liver tissue. 1.GAPDH Monoclonal Antibody(2B8)(red) was diluted at 1:200(4°C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of Human-colon tissue. 1.GAPDH Monoclonal Antibody(2B8)(red) was diluted at 1:200(4°C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1.GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1.GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Application Data
Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates. primary antibody was diluted at 1:5000. 4° over night. secondary antibody(cat: SA0438 was diluted at 1:10000. 37° 1hour.
Application Data
Chen. Luchao. et al. "Effects of purified Omphalia lapidescens protein on metastasis. cell cycle. apoptosis and the JAK-STAT signaling pathway in SGC-7901 human gastric cells." Oncology letters 15.4 (2018): 4161-4170.
Application Data
Du. Shaobo. et al. "Lycium barbarum Polysaccharides Protect Rat Corneal Epithelial Cells against Ultraviolet B-Induced Apoptosis by Attenuating the Mitochondrial Pathway and Inhibiting JNK Phosphorylation." BioMed research international 2017 (2017).
Application Data
Zhang. Chengke. et al. "Induction of size-dependent breakdown of blood-milk barrier in lactating mice by TiO2 nanoparticles." PloS one 10.4 (2015): e0122591.
Application Data
Wei. Yuzhen. et al. "CD4+ CD25+ GARP+ regulatory T cells display a compromised suppressive function in patients with dilated cardiomyopathy." Immunology 151.3 (2017): 291-303.
Application Data
Ho. Philip Wing-Lok. et al. "Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA)." Autophagy 16.2 (2020): 347-370.
Application Data
Wang. Yingying. et al. "p75NTR−/− mice exhibit an alveolar bone loss phenotype and inhibited PI3K/Akt/β‐catenin pathway." Cell proliferation 53.4 (2020): e12800.
Application Data
Immunofluorescence analysis of Hela cell. 1.Cyclin D1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GAPDH Monoclonal Antibody(2B8)(green) was diluted at 1:200(4° overnight). 2. Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature. 50min).
Application Data
Cheng. Xiaocheng. et al. "TNAP is a novel regulator of cardiac fibrosis after myocardial infarction by mediating TGF-β/Smads and ERK1/2 signaling pathways." EBioMedicine 67 (2021): 103370.
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Primary Antibodies

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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