Sign In/Register 0

We use cookies, check our Privacy Policies.

}
> Products > Primary Antibodies > β-actin Monoclonal Antibody(5B7)

β-actin Monoclonal Antibody(5B7)

Overview Description
Datasheet SDS
   Structural Proteins   
Catalog No : N1292
  • Reactivity
    Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Insect
  • Applications
    IF/ICC,WB,IHC-p
  • Modification
    Unmodfied/ Total
  • Source
    Monoclonal Mouse
  • Storage and Stability
    -20°C/1 year
  • Size
    100 ul
  • Price/Quantity
    $200
  • Quantity
Add to Cart
Details
  • Product Name
    β-actin Monoclonal Antibody(5B7)
  • Description
    actin beta(ACTB)Homo sapiens. This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
  • Catalog No
    N1292
  • Reactivity
    Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Insect
  • Applications
    IF/ICC,WB,IHC-p
  • Modification
    Unmodfied/ Total
  • Source
    Monoclonal Mouse
  • Dilution
    IF: 1:50-200 WB: 1:5000-20000 IHC: 1:200-300
  • Purification
    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actin
  • Molecular Weight (Da)
    41737
  • Gene Name
    ACTB
  • Protein Name
    Actin cytoplasmic 1
  • Human Gene ID
    60
  • Human Swiss Prot No.
    P60709
  • Immunogen
    Synthetic Peptide of β-actin
  • Specificity
    The antibody detects endogenous β-actin protein.
  • Formulation
    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
dfsdfdfd
References: sdfdsfdsf
Abstract:

dsfdsf

View Publication
Application Data
IHC Staining of Human ovary tissue. diluted at 1:200
Application Data
Western blot analysis of Hela (1) Rat brain (2) Mouse brain (3) Chicken lung (4) and Rabbit testis (5). diluted at 1:10000.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1.β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1.β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1.β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Application Data
Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates. primary antibody was diluted at 1:5000. 4° over night. secondary antibody(cat: SA0438 was diluted at 1:10000. 37° 1hour.
Application Data
Song. Zonggong. et al. "miR-352 participates in the regulation of trypsinogen activation in pancreatic acinar cells by influencing the function of autophagic lysosomes." Oncotarget9.13 (2018): 10868.
Application Data
Wen. Jiexia. et al. "Essential role of Na+/Ca2+ exchanger 1 in smoking-induced growth and migration of esophageal squamous cell carcinoma." Oncotarget 7.39 (2016): 63816.
Application Data
Wang. Jun-Jie. et al. "Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment." Neural regeneration research 14.3 (2019): 452.
Application Data
Xu. Yingming. et al. "The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain." PloS one 13.7 (2018): e0201021.
Application Data
Liu. Yanmei. et al. "Cancer Stem Cells are Regulated by STAT3 Signalling in Wilms Tumour." Journal of Cancer 9.8 (2018): 1486.
Application Data
Tao. Yuquan. et al. "Huaier Augmented the Chemotherapeutic Sensitivity of Oxaliplatin via Downregulation of YAP in Hepatocellular Carcinoma." Journal of Cancer 9.21 (2018): 3962.
Application Data
Liu. Chang. et al. "Mesenchymal Stem Cells Promote the Osteogenesis in Collagen-Induced Arthritic Mice through the Inhibition of TNF-α." Stem cells international 2018 (2018).
Application Data
Wang. Wu M.. et al. "MicroRNA-182 regulates neurite outgrowth involving the PTEN/AKT pathway." Frontiers in cellular neuroscience 11 (2017): 96.
Application Data
Tang. Qiusha. et al. "Combination of PEI-Mn0. 5Zn0. 5Fe2O4 nanoparticles and pHsp 70-HSV-TK/GCV with magnet-induced heating for treatment of hepatoma." International journal of nanomedicine 10 (2015): 7129.
Application Data
Bai. Jingchao. et al. "HSP 90 inhibitor AUY 922 can reverse Fulvestrant induced feedback reaction in human breast cancer cells." Cancer science 108.6 (2017): 1177-1184.
Application Data
Zhuang. Hao. et al. "Glycine decarboxylase induces autophagy and is downregulated by miRNA-30d-5p in hepatocellular carcinoma." Cell death & disease 10.3 (2019).
Application Data
Immunofluorescence analysis of Hela cell. 1.Cyclin A Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-actin Monoclonal Antibody(5B7)(green) was diluted at 1:200(4° overnight). 2. Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature. 50min).
Application Data
Kang. Tze Zhen Evangeline. et al. "The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer." Journal of Biological Chemistry 296 (2021).
Application Data
Wang. Yue. et al. "Fetal exposure to dichloroacetic acid and impaired cognitive function in the adulthood." Brain and Behavior 10.10 (2020): e01801.
Publications

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
    © 2024 AssayBiotech. All Rights Reserved.
    Follow Us
    Terms of Use Privacy Policy