Wnt family member 5A(WNT5A) Homo sapiens. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012],
Catalog No
N0650
Reactivity
Human
Applications
WB,IHC-p,IF/ICC,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
WNT5A; Protein Wnt-5a
Molecular Weight (Da)
Gene Name
WNT5A
Protein Name
Protein Wnt-5a
Human Gene ID
7474
Human Swiss Prot No.
P41221
Immunogen
Purified recombinant fragment of Wnt-5a expressed in E. Coli.
Specificity
Wnt-5a Monoclonal Antibody detects endogenous levels of Wnt-5a protein.
Immunohistochemistry analysis of paraffin-embedded human lung cancer (A). thyroid cancer (B). lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization with DAB staining using Wnt-5a Monoclonal Antibody.
