X-ray repair cross complementing 4(XRCC4)Homo sapiens. The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants. [provided by RefSeq, Dec 2015],
Catalog No
N1351
Reactivity
Human
Applications
WB,IHC-p,IF/ICC,IP
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:2000 IP:1:200 IF 1:200 IHC 1:50-300
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
XRCC4; DNA repair protein XRCC4; X-ray repair cross-complementing protein 4
Molecular Weight (Da)
38287
Gene Name
XRCC4
Protein Name
DNA repair protein XRCC4
Human Gene ID
7518
Human Swiss Prot No.
Q13426
Immunogen
Synthetic Peptide of XRCC4
Specificity
The antibody detects endogenous XRCC4 proteins.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1.XRCC4 Monoclonal Antibody(5C10) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Hela cell. 1.Bak Polyclonal Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2. Goat Anti Rabbit Alexa Fluor 488 Catalog:SA0665 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:SA0834 was diluted at 1:1000(room temperature. 50min).
