FAK (Phospho-Tyr397) Cell-Based ELISA Kit (CBP2067)

FAK (Phospho-Tyr397) Colorimetric Cell-Based ELISA Kit

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: CBP2067

Reactivity:Human,Mouse,Rat

Assay Type:Cell-Based ELISA

Format:Two 96-Well Plates

Range:At least 5000 cells/well

Pairing Option:Non-Phospho Antibody

Size:

Price/Quantity $500

Quantity:

Datasheet

SDS

Crystal Violet Normalization

Customizable Format

Buy One Get One

FAK (Phospho-Tyr397) Colorimetric Cell-Based ELISA Kit

An illustrated breakdown of the Colorimetric Cell-Based ELISA assay principles.

Delivery

Freight Charges

Utilize our shipping calculator at checkout to view

Lead Time

Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

Telephone

408-747-0185

Fax

408-747-0145

order@assaybiotech.com

Promotions

Product Details

Product Name

FAK (Phospho-Tyr397) Colorimetric Cell-Based ELISA Kit

Description

The FAK (Phospho-Tyr397) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect FAK (Phospho-Tyr397) protein expression profile in cells. The kit can be used for measuring the relative amounts of FAK (Phospho-Tyr397) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on FAK (Phospho-Tyr397). This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.

Catalog No

CBP2067

Format

Two 96-Well Plates

Detection Target

FAK (Phospho-Tyr397)

Reactivity

Human, Mouse, Rat

Detection Method

Colorimetric 450 nm

Assay Type

Cell-Based ELISA

SwissProt ID

Q05397

NCBI Gene ID

5747

Target Synonyms

PTK2; FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK

Storage and Stability Guarantee

4°C/6 Months

Sample Type

Researchers plate their cell line of choice

Range

At least 5000 cells/well

Time

4.5 hours