Phospho-IKK-α/β (Ser180/181) Colorimetric Cell-Based ELISA Kit
Catalog No :
CBP1126
Reactivity
H:S180/S181,M:S180/S181,R:S180/S181
Assay Type
Cell-Based ELISA
Format
2 Plates: TC 96-Well Plates
Dynamic Range
At least 5000 cells/well
Size
Price/Quantity
$500
Quantity
Details
Product Name
Phospho-IKK-α/β (Ser180/181) Colorimetric Cell-Based ELISA Kit
Description
The Phospho-IKK-α/β(Ser180/181) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-IKK-α/β(Ser180/181) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-IKK-α/β(Ser180/181) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-IKK-α/β(Ser180/181).
Catalog No
CBP1126
Format
2 Plates: TC 96-Well Plates
Detection Target
Phospho-IKK-α/β(Ser180/181)
Reactivity
H:S180/S181, M:S180/S181, R:S180/S181
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
O15111/O14920
NCBI Gene ID
1147/3551
Target Synonyms
CHUK; Conserved helix-loop-helix ubiquitous kinase; I kappa-B kinase alpha; I-kappa-B kinase 1; IkappaB kinase; IkBKA; IKK-A; IKK-alpha; IKK1; IKKA; Inhibitor of nuclear factor kappa-B kinase alpha subunit; kinase IKK-alpha; NFKBIKA; Nuclear factor NFkappaB inhibitor kinase alpha; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK-B; IKK-beta; IKK2; IKKB; kinase IKK-beta; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta
Storage and Stability Guarantee
4°C/6 Months
Sample Type
Researchers plate their cell line of choice
Range
At least 5000 cells/well
Assay Time
4.5 hours
References:
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Abstract:
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
An illustrated breakdown of the Colorimetric Cell-Based ELISA assay principles.
Western blot analysis of extracts from Jurkat cells
Western blot analysis of extracts from HepG2 cells treated with TNF 20ng/ml 5', using IKK-alpha/beta (Phospho-Ser180/181) Antibody.