The Phospho-IKK-α(Ser176)/IKK-β(Ser177) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-IKK-α(Ser176)/IKK-β(Ser177) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-IKK-α(Ser176)/IKK-β(Ser177) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-IKK-α(Ser176)/IKK-β(Ser177).
Western blot analysis of extracts from NIH-3T3 cells treated with TNF 20ng/ml 30', using IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody.