This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated Protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]
Catalog No
A0440
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Inhibitor of nuclear factor kappa-B kinase subunit alpha
Human Gene ID
1147/3551
Human Swiss Prot No.
O15111/O14920
Immunogen
The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta around the phosphorylation site of Ser180/181. AA range:151-200
Specificity
Phospho-IKKα/β (S180/181) Polyclonal Antibody detects endogenous levels of IKKα/β protein only when phosphorylated at S180/181.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HepG2 cells treated with TNF 20ng/ml 5', using IKK-alpha/beta (Phospho-Ser180/181) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human heart, using IKK-alpha/beta (Phospho-Ser180/181) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IKK-alpha/beta (Phospho-Ser180/181) Antibody