IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody (P12-1120)

IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: P12-1120

Reactivity:Human,Mouse,Rat

Applications:WB,IHC,ELISA

Modification:Phospho Specific

Clonality:Polyclonal

Storage and Stability:Stable at -20°C for at least 1 year.

Size:

Price/Quantity $205

Quantity:

Datasheet

SDS

IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody

Western blot analysis of lysates from NIH/3T3 cells treated with TNF 20ng/ml 30', using IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody.

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Lead Time

Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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408-747-0185

Fax

408-747-0145

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Product Details

Product Name

IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody

Description

Catalog No

P12-1120

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Modification

Phospho Specific

Source

Rabbit Polyclonal

Application Dilutions

WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Concentration

1 mg/ml

Storage and Stability

Stable at -20°C for at least 1 year.

Synonyms

CHUK; Conserved helix-loop-helix ubiquitous kinase; I kappa-B kinase alpha; I-kappa-B kinase 1; IkappaB kinase; IkBKA; IKK-A; IKK-alpha; IKK1; IKKA; Inhibitor of nuclear factor kappa-B kinase alpha subunit; kinase IKK-alpha; NFKBIKA; Nuclear factor NFkapp

MW (kDa)

84 kDa

NCBI Gene Symbol

CHUK/IKBKB

Gene ID

1147/3551

Swiss-Prot No.

O15111/O14920

Immunogen

The antiserum was produced against synthesized phospho-peptide derived from human IKK-alpha around the phosphorylation site of Ser176 and IKK-beta around the phosphorylation site of Ser177.

Immunogen Range

Specificity

IKK-alpha (Phospho-Ser176)/IKK-beta (Phospho-Ser177) Antibody detects endogenous levels of CHUK/IKBKB only when IKK-alpha phosphorylated at Ser176 and IKK-beta phosphorylated at Ser177.

Physical Form

Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.