IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody

Human, Mouse, Rat

WB, IHC, ELISA

Phospho Specific

Rabbit Polyclonal

WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

1 mg/ml

Stable at -20°C for at least 1 year.

CHUK; Conserved helix-loop-helix ubiquitous kinase; I kappa-B kinase alpha; I-kappa-B kinase 1; IkappaB kinase; IkBKA; IKK-A; IKK-alpha; IKK1; IKKA; Inhibitor of nuclear factor kappa-B kinase alpha subunit; kinase IKK-alpha; NFKBIKA; Nuclear factor NFkapp

84 kDa

CHUK/IKBKB

1147/3551

O15111/O14920

The antiserum was produced against synthesized phospho-peptide derived from human IKK-alpha around the phosphorylation site of Ser176 and IKK-beta around the phosphorylation site of Ser177.

IKK-alpha (Phospho-Ser176)/IKK-beta (Phospho-Ser177) Antibody detects endogenous levels of CHUK/IKBKB only when IKK-alpha phosphorylated at Ser176 and IKK-beta phosphorylated at Ser177.

Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.