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Phospho-p44/42 MAP Kinase (Thr202) Colorimetric Cell-Based ELISA Kit
Phospho-p44/42 MAP Kinase (Thr202) Colorimetric Cell-Based ELISA Kit
Catalog No :
CBP1544
Reactivity
H:T202/185,M:T203/183,R:T203/183
Assay Type
Cell-Based ELISA
Format
2 Plates: TC 96-Well Plates
Dynamic Range
At least 5000 cells/well
Size
Price/Quantity
$500
Quantity
Details
Product Name
Phospho-p44/42 MAP Kinase (Thr202) Colorimetric Cell-Based ELISA Kit
Description
The Phospho-p44/42MAPKinase(Thr202) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-p44/42MAPKinase(Thr202) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-p44/42MAPKinase(Thr202) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-p44/42MAPKinase(Thr202).
Catalog No
CBP1544
Format
2 Plates: TC 96-Well Plates
Detection Target
Phospho-p44/42MAPKinase(Thr202)
Reactivity
H:T202/185, M:T203/183, R:T203/183
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
P27361/P28482
NCBI Gene ID
5595/5594
Target Synonyms
ERK-1; ERK1; ERT2; Extracellular signal- regulated kinase 1; Insulin-stimulated MAP2 kinase; kinase ERK1; MAP kinase 1; MAPK 1; MAPK3; Microtubule- associated protein-2 kinase; Mitogen-activated protein kinase 3; MK03; MNK1; p44-ERK1; P44-ERK1; p44-MAPK; P44-MAPK; PRKM3
Storage and Stability Guarantee
4°C/6 Months
Sample Type
Researchers plate their cell line of choice
Range
At least 5000 cells/well
Assay Time
4.5 hours
References:
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Abstract:
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
An illustrated breakdown of the Colorimetric Cell-Based ELISA assay principles.
Western blot analysis of extracts from 293 cells, treated with EGF 200 ng/ml 30'
Western blot analysis of extracts from NIH-3T3 cells treated with IFN 2500U/ml 30', using p44/42 MAP Kinase (Phospho-Thr202) Antibody.