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Primary Antibodies
ERK 1/2 (Phospho-Thr202/Y204) Antibody
ERK 1/2 (Phospho-Thr202/Y204) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A8229
Reactivity:Human,Mouse,Rat,Fish
Applications:IF,WB,IHC,ELISA
Modification:Phospho Specific
Source:Store at -20°C/1 year
Storage and Stability:

$205
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ERK 1/2 (Phospho-Thr202/Y204) Antibody

Immunofluorescence analysis of mouse-spleen tissue. 1,ERK 1/2 (phospho Thr202/Y204) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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Product Details
Product Name
ERK 1/2 (Phospho-Thr202/Y204) Antibody
Description
The protein encoded byThis gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008],
Catalog No
A8229
Reactivity
Human, Mouse, Rat, Fish
Source
Polyclonal, Rabbit,IgG
Modification
Phospho Specific
Applications
IF, WB, IHC, ELISA
Dilution
IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p
Molecular Weight (Da or kDa)
43kD
Gene Name
MAPK1/MAPK3
Protein Name
Mitogen-activated protein kinase 3
Human Gene ID
5595/5594
Human Swiss Prot No.
P27361 / P28482
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human ERK 1/2 (phospho Thr202/Y204)
Specificity
Phospho-ERK 1/2 (T202/Y204) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at T202 or Y204.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (11)
Circular RNA circNRIP1 promotes migration and invasion in cervical cancer by sponging miR-629-3p and regulating the PTP4A1/ERK1/2 pathway. Cell Death & Disease Cell Death Dis. 2020 May;11(5):1-15 WB Human HeLa cell,SiHa cell

Author: Available in link provided

Date of Publication : Available in link provided

Effect of cold stress on the MAPK pathway and lipidomics on muscle of Takifugu fasciatus. AQUACULTURE Aquaculture. 2021 Jul;540:736691 WB Fish 1:1500 Muscles

Author: Available in link provided

Date of Publication : Available in link provided

Preventative effects of resveratrol and estradiol on streptozotocin-induced diabetes in ovariectomized mice and the related mechanisms. PLoS One 2018 Oct 01 WB Mouse Skeletal muscle,myocardial tissues

Author: Available in link provided

Date of Publication : Available in link provided

Novel paeonol derivatives: Design, synthesis and anti-inflammatory activity in vitro and in vivo. BIOORGANIC CHEMISTRY Bioorg Chem. 2020 May;98:103735 WB Mouse RAW264.7 cell

Author: Available in link provided

Date of Publication : Available in link provided

Negative Regulation of Grb10 Interacting GYF Protein 2 on Insulin-Like Growth Factor-1 Receptor Signaling Pathway Caused Diabetic Mice Cognitive Impairment. PLoS One 2014 Sep 30 WB Mouse 1∶1000 hippocampus

Author: Available in link provided

Date of Publication : Available in link provided

CTGF enhances resistance to 5-FU-mediating cell apoptosis through FAK/MEK/ERK signal pathway in colorectal cancer. OncoTargets and Therapy Oncotargets Ther. 2016; 9: 7285–7295 WB Human 1:500 HT-29 cell, SW620 cell

Author: Available in link provided

Date of Publication : Available in link provided

Aβ40 Promotes the Osteoblastic Differentiation of Aortic Valve Interstitial Cells through the RAGE Pathway. Current Medical Science 2020 Oct 29 WB Human 1:500 Acute aortic aortic valve interstitial cells (AVICs)

Author: Available in link provided

Date of Publication : Available in link provided

SCF/C-KIT signaling modulates tryptase expression in acute myeloid leukemia cells. INTERNATIONAL JOURNAL OF HEMATOLOGY Int J Hematol. 2014 Jun;99(6):750-757 WB Human U937 cell

Author: Available in link provided

Date of Publication : Available in link provided

Effect of the Pulsatilla decoction n-butanol extract on vulvovaginal candidiasis caused by Candida glabrata and on its virulence factors FITOTERAPIA Jiaping Zhang IF,WB Mouse 1:200 vaginal tissue

Author: Available in link provided

Date of Publication : Available in link provided

Bruceantinol works as a CDK2/4/6 inhibitor to inhibit the growth of breast cancer cells CHEMICO-BIOLOGICAL INTERACTIONS Li Sun WB Human MDA-MB-231 cell

Author: Available in link provided

Date of Publication : Available in link provided

Coptidis rhizoma extract alleviates oropharyngeal candidiasis by gC1qR-EGFR/ERK/c-fos axis-induced endocytosis of oral epithelial cells JOURNAL OF ETHNOPHARMACOLOGY Mengyuan Bao WB Human FaDu cell

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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