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Phospho-p44/42 MAP Kinase (Tyr204) Fluorometric Cell-Based ELISA Kit
Phospho-p44/42 MAP Kinase (Tyr204) Fluorometric Cell-Based ELISA Kit
Catalog No :
FLUO-CBP1545
Reactivity
H:Y204/187,M:Y205/185,R:Y205/185
Assay Type
Phospho Cell-Based ELISA
Format
2 Plates: TC 96-Well Plates
Dynamic Range
At least 5000 cells/well
Size
Price/Quantity
$500
Quantity
Details
Product Name
Phospho-p44/42 MAP Kinase (Tyr204) Fluorometric Cell-Based ELISA Kit
Description
The Phospho-p44/42 MAP Kinase (Tyr204) Fluorometric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Phospho-p44/42 MAP Kinase (Tyr204) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Phospho-p44/42 MAP Kinase (Tyr204) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CAV1 phosphorylation.
Catalog No
FLUO-CBP1545
Format
2 Plates: TC 96-Well Plates
Detection Target
p44/42 MAP Kinase (Phospho-Tyr204)
Reactivity
H:Y204/187, M:Y205/185, R:Y205/185
Detection Method
Fluorometric
Assay Type
Phospho Cell-Based ELISA
SwissProt ID
P27361/P28482
NCBI Gene ID
MAPK1/MAPK3
Target Synonyms
ERK-1; ERK1; ERT2; Extracellular signal- regulated kinase 1; Insulin-stimulated MAP2 kinase; kinase ERK1; MAP kinase 1; MAPK 1; MAPK3; Microtubule- associated protein-2 kinase; Mitogen-activated protein kinase 3; MK03; MNK1; p44-ERK1; P44-ERK1; p44-MAPK; P44-MAPK; PRKM3
Storage and Stability Guarantee
4°C/6 Months
Sample Type
Researchers plate their cell line of choice
Range
At least 5000 cells/well
Assay Time
4.5 hours
References:
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Abstract:
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Western blot analysis of extracts from HeLa cells
Western blot analysis of extracts from HeLa cells treated with EGF 200ng/ml 30', using p44/42 MAP Kinase (Phospho-Tyr204) Antibody.
