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Primary Antibodies
FLAG-tag Antibody
FLAG-tag Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: C40009
Reactivity:Species independent
Applications:WB,ELISA,IP,IF
Modification:Unmodified/Total
Source:Polyclonal,Rabbit,IgG
Storage and Stability:Store at -20°C/1 year

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FLAG-tag Antibody

Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody(catalog

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Product Details
Product Name
FLAG-tag Antibody
Description
The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Catalog No
C40009
Reactivity
Species independent
Source
Polyclonal, Rabbit,IgG
Modification
Unmodified/Total
Applications
WB, ELISA, IP, IF
Dilution
WB 1:1000 - 1:3000. ELISA: 1:5000-20000.. IF 1:50-200
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag
Molecular Weight (Da or kDa)
Gene Name
Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
Protein Name
Human Gene ID
Human Swiss Prot No.
Immunogen
DDDDK synthetic peptide conjugated to KLH.
Specificity
FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (4)
Positive feedback loop of YB-1 interacting with Smad2 promotes liver fibrosis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2017 Mar;484:753 WB Human LX-2 cell,293T cell

Author: Available in link provided

Date of Publication : Available in link provided

Pathogenic variants of meiotic double strand break (DSB) formation genes PRDM9 and ANKRD31 in premature ovarian insufficiency. GENETICS IN MEDICINE Genet Med. 2021 Jul;23(12):2309-2315 WB，IHC Human HEK293 cell

Author: Available in link provided

Date of Publication : Available in link provided

Impaired CBS-H2S signaling axis contributes to MPTP-induced neurodegeneration in a mouse model of Parkinson’s disease. BRAIN BEHAVIOR AND IMMUNITY Brain Behav Immun. 2018 Jan;67:77 WB Mouse striatum

Author: Available in link provided

Date of Publication : Available in link provided

The role of p21-activated kinase 4 in the progression of oral squamous cell carcinoma by targeting PI3K–AKT signaling pathway Clinical & Translational Oncology Fayu Liu WB Human oral squamous carcinoma cell

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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