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> Products > Primary Antibodies > LC3B Antibody

LC3B Antibody

Overview Description
Datasheet SDS
   Kinases and Phosphatases   
Catalog No : C40132
  • Reactivity
    Human,Mouse,Rat
  • Applications
    WB,IF/ICC,IHC-p
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
Add to Cart
Details
  • Product Name
    LC3B Antibody
  • Description
    The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of Cytoplasmic component. [provided by RefSeq, Jul 2008]
  • Catalog No
    C40132
  • Reactivity
    Human, Mouse, Rat
  • Applications
    WB, IF/ICC, IHC-p
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    WB: 1:1000-2000 IHC: 1:200-500 IF 1:200
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
  • Storage and Stability
    -20°C/1 year
  • Other Name
    MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta; YM3381
  • Molecular Weight (Da)
  • Gene Name
    MAP1LC3B
  • Protein Name
    Microtubule-associated proteins 1A/1B light chain 3B
  • Human Gene ID
    81631
  • Human Swiss Prot No.
    Q9GZQ8
  • Immunogen
    Recombinant Protein of MAP LC3β
  • Specificity
    The antibody detects endogenous MAP LC3β protein.
  • Formulation
    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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View Publication
Application Data
Feng, Qin. "Quantitative proteomic analysis reveals that Arctigenin alleviates concanavalin A-induced hepatitis through suppressing immune system and regulating autophagy." Frontiers in immunology 9 (2018): 1881.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat BrainTissue using MAP LC3β Polyclonal Antibody.
Application Data
Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue using MAP LC3β Polyclonal Antibody. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
Application Data
Immunofluorescence analysis of Mouse-lung tissue. 1,LC3B Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Western blot analysis of lysates from 1) Hela, 2) 3T3, 3) Rat Brain Tissue cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:SA0439)was diluted at 1:10000, 37° 1hour. （Red） GAPDH Monoclonal Antibody(2B8) (cat:N1293) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:SA0436)was diluted at 1:10000, 37° 1hour.
Application Data
Immunofluorescence analysis of Hela cell. 1,Cleaved-PARP-1 (D214) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). LC3B Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature, 50min).
Application Data
Jia, Wei‐Qiang, et al. "Verbascoside inhibits progression of glioblastoma cells by promoting Let‐7g‐5p and down‐regulating HMGA2 via Wnt/beta‐catenin signalling blockade." Journal of cellular and molecular medicine 24.5 (2020): 2901-2916.
Publications

Primary Antibodies

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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