eukaryotic translation initiation factor 4E binding protein 1(EIF4EBP1)Homo sapiens. This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008],
Catalog No
N0002
Reactivity
Human
Applications
WB,IHC-p,IF(paraffin section),ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Molecular Weight (Da)
Gene Name
EIF4EBP1
Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
Human Gene ID
1978
Human Swiss Prot No.
Q13541
Immunogen
Purified recombinant fragment of 4E-BP1 expressed in E. Coli.
Specificity
4E-BP1 Monoclonal Antibody detects endogenous levels of 4E-BP1 protein.
Immunohistochemistry analysis of paraffin-embedded human pancreas carcinoma (A). esophagus carcinoma tissue (B) and ovary tumor tissue (C). showing cytoplasmic and membrane localization with DAB staining using 4E-BP1 Monoclonal Antibody.
