apoptosis inducing factor, mitochondria associated 1(AIFM1) Homo sapiens. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome
Catalog No
N0017
Reactivity
Human,Mouse,Rat,Monkey
Applications
WB,IHC-p,IF/ICC,FCM,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
AIFM1; AIF; PDCD8; Apoptosis-inducing factor 1; mitochondrial; Programmed cell death protein 8
Molecular Weight (Da)
Gene Name
AIFM1
Protein Name
Apoptosis-inducing factor 1 mitochondrial
Human Gene ID
9131
Human Swiss Prot No.
O95831
Immunogen
Purified recombinant fragment of human AIF-M1 expressed in E. Coli.
Specificity
AIF-M1 Monoclonal Antibody detects endogenous levels of AIF-M1 protein.
Immunofluorescence analysis of NIH/3T3 cells using AIF-M1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western Blot analysis using AIF-M1 Monoclonal Antibody against NIH/3T3 (1). Jurkat (2). HeLa (3). HepG2 (4). MOLT4 (5). C6 (6). RAJI (7). Cos7 (8) and PC-12 (9) cell lysate.
