BMI1 proto-oncogene, polycomb ring finger(BMI1)Homo sapiens. This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015],
Catalog No
N0071
Reactivity
Human
Applications
WB,IHC-p,IF/ICC,FCM,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
BMI1; PCGF4; RNF51; Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51
Molecular Weight (Da)
Gene Name
BMI1
Protein Name
Polycomb complex protein BMI-1
Human Gene ID
648
Human Swiss Prot No.
P35226
Immunogen
Purified recombinant fragment of human Bmi-1 expressed in E. Coli.
Specificity
Bmi-1 Monoclonal Antibody detects endogenous levels of Bmi-1 protein.
Immunofluorescence analysis of Hela cells using Bmi-1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
