catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 on 'Ser-45'.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer. Interacts with the Axin complex.,
Catalog No
N0091
Reactivity
Human
Applications
WB,IHC-p,IF(paraffin section),ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1
Molecular Weight (Da)
Gene Name
CSNK1A1
Protein Name
Casein kinase I isoform alpha
Human Gene ID
1452
Human Swiss Prot No.
P48729
Immunogen
Purified recombinant fragment of Casein Kinase Iα expressed in E. Coli.
Immunohistochemistry analysis of paraffin-embedded human liver tissue (A). colon carcinoma (B). lung carcinoma (C) and esophagus tissue (D). showing membrane localization with DAB staining using Casein Kinase Iα Monoclonal Antibody.
