chitinase 1(CHIT1) Homo sapiens. Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012],
Catalog No
N0151
Reactivity
Human
Applications
WB,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
CHIT1; Chitotriosidase-1; Chitinase-1
Molecular Weight (Da)
Gene Name
CHIT1
Protein Name
Chitotriosidase-1
Human Gene ID
1118
Human Swiss Prot No.
Q13231
Immunogen
Purified recombinant fragment of Chitotriosidase (aa22-137) expressed in E. Coli.
Specificity
Chitotriosidase Monoclonal Antibody detects endogenous levels of Chitotriosidase protein.
Western Blot analysis using Chitotriosidase Monoclonal Antibody against truncated Trx-CHIT1 recombinant protein (1) and truncated CHIT1 (aa22-466)-hIgGFc transfected CHO-K1 cell lysate (2).
