crystallin alpha B(CRYAB)Homo sapiens. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distin
Catalog No
N0168
Reactivity
Human
Applications
WB,IHC-p,IF(paraffin section),ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
CRYAB; CRYA2; Alpha-crystallin B chain; Alpha(B)-crystallin; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
Molecular Weight (Da)
Gene Name
CRYAB
Protein Name
Alpha-crystallin B chain
Human Gene ID
1410
Human Swiss Prot No.
P02511
Immunogen
Purified recombinant fragment of Crystallin-αB (aa1-175) expressed in E. Coli.
Specificity
Crystallin-αB Monoclonal Antibody detects endogenous levels of Crystallin-αB protein.
Immunohistochemistry analysis of paraffin-embedded human brain hippocampus (A). lung cancer (B). brain tumor (C). breast cance (D). showing cytoplasmic localization with DAB staining using Crystallin-αB Monoclonal Antibody.
Western Blot analysis using Crystallin-αB Monoclonal Antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CRYAB cDNA (2).
