Description
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.,PTM:Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Mainly cytoplasmic, also present in lipid rafts.,subunit:Interacts with PTPN8 (By similarity). Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1.,tissue specificity:Expressed in lung and macrophages.,
Reactivity
Human,Mouse,Rat,Monkey
Applications
WB,IF/ICC,FCM,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
CSK; Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL
Molecular Weight (Da)
Gene Name
CSK
Protein Name
Tyrosine-protein kinase CSK
Human Gene ID
1445
Human Swiss Prot No.
P41240
Immunogen
Purified recombinant fragment of human Csk expressed in E. Coli.
Specificity
Csk Monoclonal Antibody detects endogenous levels of Csk protein.
Formulation
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
