cyclin B1(CCNB1)Homo sapiens. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008],
Catalog No
N0174
Reactivity
Human,Rat
Applications
WB,IF/ICC,FCM,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
CCNB1; CCNB; G2/mitotic-specific cyclin-B1
Molecular Weight (Da)
Gene Name
CCNB1
Protein Name
G2/mitotic-specific cyclin-B1
Human Gene ID
891
Human Swiss Prot No.
P14635
Immunogen
Purified recombinant fragment of human Cyclin B1 expressed in E. Coli.
Immunofluorescence analysis of Hela cells using Cyclin B1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
