Description
DEAD-box helicase 4(DDX4)Homo sapiens. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009],
Reactivity
Human
Applications
WB,IHC-p,IF/ICC,FCM,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
DDX4; VASA; Probable ATP-dependent RNA helicase DDX4; DEAD box protein 4; Vasa homolog
Molecular Weight (Da)
Gene Name
DDX4
Protein Name
Probable ATP-dependent RNA helicase DDX4
Human Gene ID
54514
Human Swiss Prot No.
Q9NQI0
Immunogen
Purified recombinant fragment of human DDX4 expressed in E. Coli
Specificity
DDX4 Monoclonal Antibody detects endogenous levels of DDX4 protein.
Formulation
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
