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> Products > Primary Antibodies > β-Tubulin Monoclonal Antibody(5G3)

β-Tubulin Monoclonal Antibody(5G3)

Overview Description
Datasheet SDS
   Structural Proteins   
Catalog No : N1294
  • Reactivity
    Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Sheep,Insect,Yeast,Fish
  • Applications
    WB,IF/ICC,IHC-p
  • Modification
    Unmodfied/ Total
  • Source
    Monoclonal Mouse
  • Storage and Stability
    -20°C/1 year
  • Size
    100 ul
  • Price/Quantity
    $200
  • Quantity
Add to Cart
Details
  • Product Name
    β-Tubulin Monoclonal Antibody(5G3)
  • Description
    tubulin beta 3 class III(TUBB3)Homo sapiens. This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010],
  • Catalog No
    N1294
  • Reactivity
    Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Sheep,Insect,Yeast,Fish
  • Applications
    WB,IF/ICC,IHC-p
  • Modification
    Unmodfied/ Total
  • Source
    Monoclonal Mouse
  • Dilution
    WB: 1:5000 IHC: 1:200 IF 1:200
  • Purification
    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III
  • Molecular Weight (Da)
    50433
  • Gene Name
    TUBB3
  • Protein Name
    Tubulin beta-3 chain
  • Human Gene ID
    10381
  • Human Swiss Prot No.
    Q13509
  • Immunogen
    Synthetic Peptide of β-Tubulin
  • Specificity
    The antibody detects endogenous β-tubulin protein.
  • Formulation
    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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Application Data
IF analysis of Hela with β-Tubulin Monoclonal Antibody(Left) and DAPI (Right) diluted at 1:100.
Application Data
IHC Staining of Human colon tissue. diluted at 1:200.
Application Data
Western blot analysis of A549 (1). Rat brain (2). Mouse brain (3). Chicken lung (4) and Rabbit testis (5). Sheep muscle (6). diluted at 1:5000.
Application Data
Immunofluorescence analysis of Mouse-lung tissue. 1.β-Tubulin Monoclonal Antibody(5G3)(red) was diluted at 1:200(4°C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of Human-appendix tissue. 1.β-Tubulin Monoclonal Antibody(5G3)(red) was diluted at 1:200(4°C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1.β-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.β-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1.β-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Application Data
Meng. Yujiao. et al. "Paeonol ameliorates imiquimod-induced psoriasis-like skin lesions in BALB/c mice by inhibiting the maturation and activation of dendritic cells." International journal of molecular medicine 39.5 (2017): 1101-1110.
Application Data
Diao. Zhiqing. et al. "SIRT3 consolidates heterochromatin and counteracts senescence." Nucleic acids research 49.8 (2021): 4203-4219.
Application Data
Cheng. X.. Wang. J.. Liu. C. et al. Zinc transporter SLC39A13/ZIP13 facilitates the metastasis of human ovarian cancer cells via activating Src/FAK signaling pathway. J Exp Clin Cancer Res 40. 199 (2021).
Application Data
Immunofluorescence analysis of Hela cell. 1.CREB-1 (phospho Ser133) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-Tubulin Monoclonal Antibody(5G3)(green) was diluted at 1:200(4° overnight). 2. Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature. 50min).
Publications

Primary Antibodies

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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