tubulin beta 3 class III(TUBB3)Homo sapiens. This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010],
Catalog No
N1400
Reactivity
Human,Mouse,Rat
Applications
WB,IF/ICC
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:5000 -10000 IF: 1:100-200
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Immunofluorescence analysis of Hela cell. 1.ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-tubulin Monoclonal Antibody(M7)(green) was diluted at 1:200(4° overnight). 2. Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature. 50min).