interleukin 2 receptor subunit alpha(IL2RA) Homo sapiens. The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009],
Catalog No
N1372
Reactivity
Human
Applications
IHC-p,IF(paraffin section)
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
IHC: 1:200
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.CD25 Monoclonal Antibody(Q22) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
