histone cluster 1 H2B family member b(HIST1H2BB)Homo sapiens. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],
Catalog No
N1427
Reactivity
Human,Mouse,Rat
Applications
WB,IF/ICC
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:1000-3000 IF 1:200
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Western blot analysis of 1) Hela. 2) 3T3. 3) Raw264.7. 4) Rat Brain. 5) Rat Kidney. diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).
Immunofluorescence analysis of Hela cell. 1.Brp44L Polyclonal Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2. Goat Anti Rabbit Alexa Fluor 488 Catalog:SA0665 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:SA0834 was diluted at 1:1000(room temperature. 50min).
