nitric oxide synthase 3(NOS3) Homo sapiens. Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
Catalog No
N1429
Reactivity
Human,Mouse,Rat
Applications
WB
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:500-2000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
NOS3; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
Molecular Weight (Da)
133289
Gene Name
NOS3
Protein Name
Nitric oxide synthase, endothelial
Human Gene ID
4846
Human Swiss Prot No.
P29474
Immunogen
Recombinant Protein of eNOS
Specificity
The antibody detects endogenous eNOS protein.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Western blot analysis of lysates from 1) Rat Heart Tissue. 2) huvec.3) Jurkat cells. (Green) primary antibody was diluted at 1:1000. 4°over night. secondary antibody(cat:SA0438)was diluted at 1:10000. 37° 1hour. (Red) Actin β Polyclonal Antibody (cat:C40022) antibody was diluted at 1:5000 as loading control. 4° over night.secondary antibody(cat:SA0437)was diluted at 1:10000. 37° 1hour.
Western blot analysis of lysates from 1) Rat Heart Tissue. 2) huvec.3) Jurkat cells. （Green） primary antibody was diluted at 1:1000. 4°over night. secondary antibody(cat:SA0438)was diluted at 1:10000. 37° 1hour. （Red） Actin β Polyclonal Antibody (cat:C40022) antibody was diluted at 1:5000 as loading control. 4° over night.secondary antibody(cat:SA0437)was diluted at 1:10000. 37° 1hour.
