catenin beta 1(CTNNB1) Homo sapiens. The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016],
Catalog No
N1514
Reactivity
Human,Mouse,Rat
Applications
WB,IF/ICC,IHC-p
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:1000-2000 IHC: 1:200-500 IF 1:200
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1.Catenin-β Monoclonal Antibody(4F2) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Catenin-β Monoclonal Antibody(4F2) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Liu. Taian. et al. "Developmental protein kinase C hyper-activation results in microcephaly and behavioral abnormalities in zebrafish." Translational psychiatry 8 (2018).
