cystatin C(CST3)Homo sapiens. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associate
Catalog No
N1608
Reactivity
Human
Applications
IF/ICC,WB,IHC-p,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CST3
Molecular Weight (Da)
Gene Name
CST3
Protein Name
CST3
Human Gene ID
1471
Human Swiss Prot No.
P01034
Immunogen
Recombinant Protein of Cystatin C of CST3
Specificity
Cystatin C protein detects endogenous levels of CST3
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemical analysis of paraffin-embedded Human heart. 1. Antibody was diluted at 1:100(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human heart. 1. Antibody was diluted at 1:100(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human heart. 1. Antibody was diluted at 1:100(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1. Antibody was diluted at 1:400(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1. Antibody was diluted at 1:400(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1. Antibody was diluted at 1:400(4° overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Cystatin C Mouse Monoclonal Antibody(7F11) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
