chromobox 5(CBX5)Homo sapiens. This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008],
Catalog No
N1687
Reactivity
Human,Mouse,Rat
Applications
IF/ICC,WB,IHC-p
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
IF: 1:50-200 WB 1:500-2000,IHC-p 1:50-300
Purification
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
Chromobox protein homolog 5 (Antigen p25) (Heterochromatin protein 1 homolog alpha) (HP1 alpha)
Molecular Weight (Da)
Gene Name
CBX5 HP1A
Protein Name
Chromobox protein homolog 5 (Antigen p25) (Heterochromatin protein 1 homolog alpha) (HP1 alpha)
Human Gene ID
23468
Human Swiss Prot No.
P45973
Immunogen
Recombinant Protein of HP-1α
Specificity
The antibody detects endogenous HP-1α protein
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.HP-1α Mouse Monoclonal Antibody(5E3) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.HP-1α Mouse Monoclonal Antibody(5E3) was diluted at 1:200(4°C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98°C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.
