keratin 5(KRT5)Homo sapiens. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008],
Catalog No
N1837
Reactivity
Human, Mouse
Applications
IHC-p,IF(paraffin section)
Modification
Unmodfied/ Total
Source
Monoclonal Mouse/IgG2a, Kappa
Dilution
IHC-p 1:100-500，WB 1:500-2000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
Molecular Weight (Da)
Gene Name
KRT5
Protein Name
Keratin, type II cytoskeletal 5 (58 kDa cytokeratin) (Cytokeratin-5) (CK-5) (Keratin-5) (K5) (Type-II keratin Kb5)
Human Gene ID
3852
Human Swiss Prot No.
P13647
Immunogen
Synthesized peptide derived from human Cytokeratin 5
Specificity
This antibody detects endogenous levels of human Cytokeratin 5. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemical analysis of paraffin-embedded human Tonsil. 1.Cytokeratin 5 Antibody was diluted at 1:200(4° overnight). 2. EDTA pH 8.0 was used for antigen retrieval
Immunohistochemical analysis of paraffin-embedded human Tonsil-High magnification. 1.Cytokeratin 5 Antibody was diluted at 1:200(4° overnight). 2. EDTA pH 8.0 was used for antigen retrieval
Immunohistochemical analysis of paraffin-embedded human Skin squamous cell carcinoma. 1.Cytokeratin 5 Antibody was diluted at 1:200(4° overnight). 2. EDTA pH 8.0 was used for antigen retrieval
Immunohistochemical analysis of paraffin-embedded human Skin squamous cell carcinoma-High magnification. 1.Cytokeratin 5 Antibody was diluted at 1:200(4° overnight). 2. EDTA pH 8.0 was used for antigen retrieval
Immunohistochemical analysis of paraffin-embedded human Medullary Thyroid Carcinoma. 1.Cytokeratin 5 Antibody was diluted at 1:200(4° overnight). 2. EDTA pH 8.0 was used for antigen retrieval
Immunohistochemical analysis of paraffin-embedded human Medullary Thyroid Carcinoma-High magnification. 1.Cytokeratin 5 Antibody was diluted at 1:200(4° overnight). 2. EDTA pH 8.0 was used for antigen retrieval
Immunohistochemical analysis of paraffin-embedded human Lung squamous cell carcinoma. 1.Cytokeratin 5 Antibody was diluted at 1:200(4° overnight). 2. EDTA pH 8.0 was used for antigen retrieval
Immunohistochemical analysis of paraffin-embedded human Lung squamous cell carcinoma-High magnification. 1.Cytokeratin 5 Antibody was diluted at 1:200(4° overnight). 2. EDTA pH 8.0 was used for antigen retrieval
Immunohistochemical analysis of paraffin-embedded human Cervical squamous cell carcinoma. 1.Cytokeratin 5 Antibody was diluted at 1:200(4° overnight). 2. EDTA pH 8.0 was used for antigen retrieval
Immunohistochemical analysis of paraffin-embedded human Cervical squamous cell carcinoma-High magnification. 1.Cytokeratin 5 Antibody was diluted at 1:200(4° overnight). 2. EDTA pH 8.0 was used for antigen retrieval
Immunohistochemical analysis of paraffin-embedded Squamous cell lung carcinoma. 1. Antibody was diluted at 1:200(4° overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
