Description
CK5 is a cytokeratin with a molecular weight of 58 kDa. In normal tissues, CK5 is expressed in squamous epithelium, germinal layer cells of some squamous epithelium, basal cells of ductal epithelium, myoepithelial cells and mesothelial cells, and almost no expression in most glandular epithelial cells. Therefore, it can be used for the differential diagnosis of squamous cell carcinoma and adenocarcinoma, mesothelioma and adenocarcinoma, as well as benign and malignant tumors of prostate, breast and salivary gland epithelium.
Reactivity
Human
Application
IHC,WB
Modification
Unmodified/ Total
Source
Monoclonal Mouse
Storage and Stability
Stable at -20°C for at least 1 year.
Other Name
58 kDa cytokeratin, CK-5, CK5, Cytokeratin-5, Cytokeratin5, DDD, DDD1, EBS2, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K2C5_HUMAN, K5, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types), Keratin 5, Keratin, keratin complex 2, basic, gene 5, keratin, type II cytoskeletal 5, Keratin-5, Keratin5, KRT 5, Krt5, KRT5A, type II cytoskeletal 5, Type-II keratin Kb5
Gene Name
KRT5
UniProtKB
P13647
Clone ID
ABT051
Isotype
IgG2a, Kappa
Localization
Cytoplasmic, Membranous
Positive Tissue Control
Tonsil/ Prostate
Specificity
The antibody can specifically recognize human CK5 protein, and shows no cross reaction with CK6. In western blotting of A431, A549 and Hela cell lysates, the antibody can label a 58 kDa band corresponding to CK5 only in A431 cell lysates.
Formulation
PBS, pH7.2, 0.03% Proclin 300, 50% glycerol
