PI 3-kinase p85α (Phospho-Tyr607) Cell-Based ELISA Kit (CBP2028)

PI 3-kinase p85α (Phospho-Tyr607) Colorimetric Cell-Based ELISA Kit

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: CBP2028

Reactivity:Human,Mouse,Rat,Chicken(testedbyourcustomer）

Assay Type:Cell-Based ELISA

Format:Two 96-Well Plates

Range:At least 5000 cells/well

Pairing Option:Non-Phospho or Total Antibody

Size:

Price/Quantity $500

Quantity:

Datasheet

SDS

Crystal Violet Normalization

Customizable Format

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PI 3-kinase p85α (Phospho-Tyr607) Colorimetric Cell-Based ELISA Kit

An illustrated breakdown of the Colorimetric Cell-Based ELISA assay principles.

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Lead Time

Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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408-747-0185

Fax

408-747-0145

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Product Details

Product Name

PI 3-kinase p85α (Phospho-Tyr607) Colorimetric Cell-Based ELISA Kit

Description

The PI 3-kinase p85α (Phospho-Tyr607) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect PI 3-kinase p85α (Phospho-Tyr607) protein expression profile in cells. The kit can be used for measuring the relative amounts of PI 3-kinase p85α (Phospho-Tyr607) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on PI 3-kinase p85α (Phospho-Tyr607). This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.

Catalog No

CBP2028

Format

Two 96-Well Plates

Detection Target

PI 3-kinase p85α (Phospho-Tyr607)

Reactivity

Human, Mouse, Rat, Chicken(testedbyourcustomer）

Detection Method

Colorimetric 450 nm

Assay Type

Cell-Based ELISA

SwissProt ID

P27986

NCBI Gene ID

5295

Target Synonyms

PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph

Storage and Stability Guarantee

4°C/6 Months

Sample Type

Researchers plate their cell line of choice

Range

At least 5000 cells/well

Time

4.5 hours