The Phospho-BCL-2(Ser70) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-BCL-2(Ser70) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-BCL-2(Ser70) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-BCL-2(Ser70).
Western blot analysis of extracts from HeLa cells treated with LPS (40nM, 30mins), K562 cells treated with calyculin A (50ng/ml, 30mins) and COS-7 cells treated with H2O2 (1ng/ml, 15mins), using BCL-2 (Phospho-Ser70) Antibody.