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> Products > Primary Antibodies > Bcl-2 Antibody

Bcl-2 Antibody

Overview Description
Datasheet SDS
   Tumor Suppressors/Apoptosis   
Catalog No : B0774
  • Reactivity
    Human,Mouse,Rat
  • Applications
    WB,IHC-p,IF/ICC,ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
Add to Cart
Details
  • Product Name
    Bcl-2 Antibody
  • Description
    BCL2, apoptosis regulator(BCL2) Homo sapiens This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
  • Catalog No
    B0774
  • Reactivity
    Human, Mouse, Rat
  • Applications
    WB, IHC-p, IF/ICC, ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    BCL2; Apoptosis regulator Bcl-2
  • Molecular Weight (Da)
    26266
  • Gene Name
    BCL2
  • Protein Name
    Apoptosis regulator Bcl-2
  • Human Gene ID
    596
  • Human Swiss Prot No.
    P10415
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human BCL-2. AA range:46-95
  • Specificity
    Bcl-2 Polyclonal Antibody detects endogenous levels of Bcl-2 protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western blot analysis of lysates from K562 cells, using BCL-2 Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using BCL-2 Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Immunofluorescence analysis of HepG2 cells, using BCL-2 Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Western Blot analysis of 3T3 cells using Bcl-2 Polyclonal Antibody diluted at 1:1000
Application Data
Western Blot analysis of various cells using Bcl-2 Polyclonal Antibody diluted at 1:1000
Application Data
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
Application Data
Western blot analysis of lysates from 1)mouse-liver, 2)mouse-brain, 3)mouse-lung, 4)mcf-7 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(ABT:SA0439)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (ABT:N1292) antibody was diluted at 1:5000 as loading control, 4° over night,Dylight 680 secondary antibody(ABT:SA0436)was diluted at 1:10000, 37° 1hour.
Application Data
Wang, Ai-Li, et al. "Glutamine ameliorates intestinal ischemia-reperfusion Injury in rats by activating the Nrf2/Are signaling pathway." International journal of clinical and experimental pathology 8.7 (2015): 7896.
Application Data
Pan, Yun, et al. "Protective effects of tanshinone IIA sodium sulfonate on ischemia-reperfusion-induced myocardial injury in rats." Iranian journal of basic medical sciences 20.3 (2017): 308.
Application Data
Jiang, Ruibin, et al. "Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells Hypoxia/Reoxygenation Injury via Effects on miR-21 Expression, PTEN/Akt, and the Bcl-2/Bax Pathway." Evidence-Based Complementary and Alternative Medicine 2018 (2018).
Application Data
Fang, Zhiqing, et al. "Simvastatin inhibits renal cancer cell growth and metastasis via AKT/mTOR, ERK and JAK2/STAT3 pathway." PloS one 8.5 (2013): e62823.
Application Data
Yang, Ya’nan, et al. "Reversing platinum resistance in ovarian cancer multicellular spheroids by targeting Bcl-2." OncoTargets and therapy 12 (2019): 897.
Application Data
Chen, Puxiang, et al. "Long noncoding RNA LINC00152 promotes cell proliferation through competitively binding endogenous miR‐125b with MCL‐1 by regulating mitochondrial apoptosis pathways in ovarian cancer." Cancer medicine 7.9 (2018): 4530-4541.
Application Data
Liu, Yanmei, et al. "Cancer Stem Cells are Regulated by STAT3 Signalling in Wilms Tumour." Journal of Cancer 9.8 (2018): 1486.
Application Data
Bai, Ding-Ping, et al. "Zinc oxide nanoparticles induce apoptosis and autophagy in human ovarian cancer cells." International journal of nanomedicine 12 (2017): 6521.
Application Data
Xu, Shuai, et al. "Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect." RNA biology12.12 (2015): 1355-1363.
Application Data
Liu, Jing-Cai, et al. "Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro." Cell death & disease 8.8 (2017): e2966.
Application Data
Xiao, Wei, et al. "Notch signaling plays a crucial role in cancer stem-like cells maintaining stemness and mediating chemotaxis in renal cell carcinoma." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 41.
Publications

Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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