BCL2, apoptosis regulator(BCL2) Homo sapiens This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Catalog No
A7026
Reactivity
Human, Rat, Mouse
Applications
WB, IHC-p, IP, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
BCL2; Apoptosis regulator Bcl-2
Molecular Weight (Da)
26266
Gene Name
BCL2
Protein Name
Apoptosis regulator Bcl-2
Human Gene ID
596
Human Swiss Prot No.
P10415
Immunogen
The antiserum was produced against synthesized peptide derived from human BCL-2 around the phosphorylation site of Thr56. AA range:26-75
Specificity
Phospho-Bcl-2 (T56) Polyclonal Antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at T56.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from K562 cells treated with H2O2 100uM 30', using BCL-2 (Phospho-Thr56) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BCL-2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using BCL-2 (Phospho-Thr56) Antibody
Bu, Xuefeng, et al. "Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress." American journal of cancer research 6.5 (2016): 924.
