CASP9 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 9 can undergo autoproteolytic processing and activation by the apoptosome, a Protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. Caspase 9 is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Catalog No
L0110
Reactivity
Human, Mouse, Rat
Applications
IF/ICC, WB, IHC-p, ELISA
Modification
Cleaved Specific
Source
Polyclonal Rabbit
Dilution
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
The antiserum was produced against synthesized peptide derived from human Caspase 9. AA range:323-372
Specificity
Cleaved-Caspase-9 (D353) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 protein resulting from cleavage adjacent to D353.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from NIH/3T3 cells, treated with Etoposide 25uM 60', using Caspase 9 (Cleaved-Asp353) Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 (Cleaved-Asp353) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Cleaved-Caspase-9 (D353) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Cleaved-Caspase-9 (D353) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Cleaved-Caspase-9 (D353) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-testis tissue. 1,Cleaved-Caspase-9 (D353) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Huang, Hui, et al. "Microcystin-LR Induced Apoptosis in Rat Sertoli Cells via the Mitochondrial Caspase-Dependent Pathway: Role of Reactive Oxygen Species." Frontiers in physiology 7 (2016): 397.